Ask Mr. Wizard

Yeast Propagation From A Bottle

TroubleShooting

Mike Conant — Aptos, California asks,
Q

If I am starting from commercial bottle dregs for yeast harvesting, Can I just pitch the dregs into a 3-L (3-qt.) starter at 1.035–1.040 that is well fed, oxygenated, and on a stir plate; or do I need to step through multiple stages to get to my target volume, like most of the literature out there tells me to? Why can’t I just target making a single, end-point volume starter, and expect ~150 billion cells per liter as an outcome?

A
I love these two part questions that begin with great fundamentals and then segue into the meaning of life. You want to propagate yeast from bottles and are attracted to skipping the
Response by Ashton Lewis.